Surface-Free Single-Molecule Force Spectroscopy
نویسندگان
چکیده
منابع مشابه
Label-free biosensing with single-molecule force spectroscopy.
We demonstrate here a novel single-molecule, label-free bioanalytical system capable of sensing the presence of specific ssDNA oligomer sequences and proteins with high selectivity and sensitivity. An ssDNA concentration of 1 nM and a Lyz concentration of 0.65 nM could be detected.
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The deflection of a cantilever is measured using a laser beam and position-sensing detector to obtain force and displacement for both imaging and force spectroscopy. In force spectroscopy, the cantilever is attached to one end of the protein or DNA sample while the other end is immobilized on the surface. The force and position of the tip are measured as the surface is moved away from the tip, ...
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The direct measurement of forces between individual receptor-ligand complexes is an emerging new field. With the atomic force microscope (AFM) it has been possible to probe the forces between a range of biological molecules, including complementary oligo-nucleotides, proteins and carbohydrate interactions. To date, the force required to rupture these single ligandreceptor interactions is common...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2015
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2014.11.1947